期刊论文

Experimental Research of Naotaifang on Expression of VEGF and Angiopoietins in Rats after Cerebral Ischemia

中文

湖南中医药大学学报

1674-070X

2016

36

1

19-22

10.3969/j.issn.1674-070X.2016.01.004

国家自然青年科学基金项目（81202794）； 中西医结合心脑疾病防治湖南省实验室开放基金项目（2014XNFZ05）； 中西医结合防治心脑疾病的相关基础研究湖南高校科技创新团队科研项目（2015CXTD）；

本校为第一机构

目的 探讨中药脑泰方对大鼠脑缺血后血内皮生长因子(vascular endothelial growth factor,VEGF)和血管生成素(angiopoietins,Ang)的表达变化及其在血管生成中的作用.方法 选择40只雄性SD大鼠,采用随机区组法分为A正常对照组,B假手术组,C MCAO模型组,D脑泰方组;,观察各组大鼠神经功能缺失计分、脑梗死体积;RT-PCR、Western blot方法检测不同组大鼠脑组织中VEGF、Ang-1及Ang-2 mRNA和蛋白的表达变化.结果 神经功能缺失计分及TTC染色显示脑泰方组脑缺血情况缓解;大鼠脑缺血后VEGF、Ang-2 的表达水平增高,脑泰方组表达显著高于模型组(P

Objective To investigate Naotaifang on expression of VEGF and angiopoietins in rats after cerebral ischemia, and its roles in angiogenesis. Methods 40 male SD rats were randomly divided into the control group (A), sham-operation group (B),MCAO model group (C),Naotaifang (NTF) group (D). The score of neurological deficiency and volume of cerebral infarction were observed. The protein expression of VEGF, Ang-1 and Ang -2 in cerebral tissues of rats were detected by RT-PCR and Western blot. Results The score of neurological evaluation and TCC staining showed that the cerebral ischemia situation o...