The HPLC method was established to determine the fingerprint of the Chinese traditional medicine Buyanghuanwu decoction. Chromatographic conditions included Krosmasil C18 column (250 × 4. 6 mm, 5 μm) and the mobile phase composed of a mixture of acetonitrile-water(gradient elution), detection wavelength at 220 nm. The mobile phase flow rate was 1.0 mL/min. Thirteen peaks were observed on the HPLC fingerprint of Buyanghuanwu decoction. The results showed that the method was reliable and could be he...