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Study of hemoglobin response to mid-ultraviolet (UVB) radiation using micro-Raman spectroscopy

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成果类型:
期刊论文
作者:
Huang, Y. Y.;Li, N.;Zhou, S. N.;Huang, Z. T.;Zhuang, Z. F.*
通讯作者:
Zhuang, Z. F.
作者机构:
[Zhuang, Z. F.; Huang, Y. Y.; Li, N.] South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.
[Zhuang, Z. F.; Huang, Y. Y.; Li, N.] South China Normal Univ, Coll Biophoton, Lab Photon Chinese Med, Guangzhou 510631, Guangdong, Peoples R China.
[Huang, Y. Y.] South China Normal Univ, Lab Photon Informat Technol, Guangzhou 510631, Guangdong, Peoples R China.
[Zhou, S. N.] Hunan Univ Chinese Med, Hosp 1, Changsha 410007, Hunan, Peoples R China.
[Huang, Z. T.] Guzhen Prod Promot Ctr, Zhongshan, Peoples R China.
通讯机构:
[Zhuang, Z. F.] S
South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.
South China Normal Univ, Coll Biophoton, Lab Photon Chinese Med, Guangzhou 510631, Guangdong, Peoples R China.
语种:
英文
期刊:
Applied Physics B: Lasers and Optics
ISSN:
0946-2171
年:
2017
卷:
123
期:
9
页码:
1-7
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11404116, 61335011, 61275187]; Young Teachers Nurturing Fund of South China Normal University [2012KJ020]; Horizontal Topic Research Project of SENSING
机构署名:
本校为其他机构
院系归属:
第一中医临床学院
摘要:
Confocal micro-Raman spectroscopy is employed to monitor the damage to haemoglobin from mid-ultraviolet (UVB) radiation. We obtained the Raman spectra of an erythrocyte, which indicated that a peroxidation reaction occurs after UVB radiation. Further, the surface enhanced Raman scattering (SERS) spectra of isolated haemoglobin show that the intensities of the 1375 and 1399 cm−1bands, which are markers of haem aggregation, obviously increase with prolonged UVB irradiation. This increase reveals that haem aggregation occurs in the peroxidation of erythrocytes. The UV–Vis spectra...

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