期刊论文

中文

中华中医药杂志

1673-1727

2014

29

8

2484-2488

湖南省中药学重点学科资助(No.湘教通[2011]76号),中药有毒物质防控技术湖南省工程实验室项目 Science and Technology Plan Projects of Hunan Province 中国博士后科学基金 China Postdoctoral Science Foundation National Natural Science Foundation of China Funded by Key Discipline Traditional Chinese Pharmacology of Hunan Province 国家自然科学基金 Toxic Substance of Traditional Chinese Medicine Prevention and Control Technology Laboratory Program of Hunan Province 湖南省科技计划项目

本校为第一机构

目的:筛选地骨皮中的过氧化物酶体增殖物激活受体1 (PPAR γ)激动剂,确定具有调节糖脂代谢作用的潜在活性成分。方法:运用PPAR γ受体表达质粒、报告质粒(tk-PPREx3-luc)与编码绿色荧光蛋白(pEGFP-Nl)质粒短时转染HEK293细胞的体外筛选模型,通过活性跟踪分离筛选地骨皮中的PPAR γ激动剂,评价配体浓度对PPAR γ活性的响应。采用与高选择性PPAR γ拮抗剂T0070907共孵育培养,分析PPAR γ对配体的依耐程度。结果:地骨皮中的脂肪酸类具有较强的PPAR γ激动活性,而芳香酸则无明显效果。脂肪酸的活性大小为:棕榈酸>亚麻酸>亚油酸>硬脂酸>油酸>蜂花酸。棕榈酸在1.0-10μg/mL范围内,浓度与PPAR γ激动活...

Objective： To screen the active component PPARγ in Cortex lycii to make sure potential active ingredients that can regulate the lipid metabolism. Methods： The human HEK-293 ceils were transfected by PPARy receptor expression, PPAR luciferase reporter plasmid （tk-PPREx3-luc） and encoding enhanced green fluorescent protein plasmids （pEGFP-N1） that were used as screening model in vitro and be hatched together with the high selectivity PPARγ, antagonist T0070907. The dependence on ligand of PPARy was analyzed. Results： The fatty acids in C...