期刊论文

中文

中药新药与临床药理

1003-9783

2016

27

1

33-38

10.3969/j.issn.1003-9783.2016.01.008

国家自然科学基金（81303004） 湖南省科技厅资助项目（2009RS3018） 湖南省教育厅资助项目（09C727）

本校为第一机构

目的通过观察左归丸对化疗致卵巢早衰(POF)模型小鼠卵巢Cx37蛋白及mRNA表达的影响,探讨左归丸治疗POF的作用与机制。方法将昆明雌性小鼠72只随机分为空白组,模型组,戊酸雌二醇片组,左归丸低、中、高剂量组。除空白组小鼠腹腔注射等量生理盐水外,其余各组小鼠均按30 mg·kg~(-1)剂量腹腔注射环磷酰胺,每天1次,连续20 d,复制POF模型;同时从造模第1天开始按剂量要求进行灌胃给药,每天1次,连续30 d。末次给药2 h后处死小鼠,摘取双侧卵巢,部分卵巢组织液氮保存。采用RT-PCR法检测卵巢Cx37 mRNA表达;采用Western-bloT法检测卵巢Cx37蛋白的表达;采用免疫组化方法检测卵巢组织Cx37蛋白的分布。结果左归丸...

Objective To observe the effect of Zuogui Pill (ZP)on the expression of ovarian gap junction Cx37 protein and mRNA in chemotherapy-induced premature ovarian failure(POF)mouse model,and to explore the mechanism. Methods Seventy-two Kunming female mice were randomly divided into blank control group,model group,estradiol valerate tables group,and low-,medium- and high-dose ZP groups. Mice of the blank control group were injected with saline intraperitoneally,and mice of the other groups were given intraperitoneal injection of cytoxan(CTX,30 mg·kg...