A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction

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成果类型：

期刊论文

作者：

Zhou, Hongyan;Tong, Chunyi;Zou, Wei;Yang, Yupei;Liu, Yongbei;...

通讯作者：

Liu, Bin;Wang, Wei

作者机构：

[Liu, Bin; Dang, Wenya; Zhou, Hongyan; Tong, Chunyi] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.

[Liu, Bin; Yang, Yupei; Qin, Yan; Li, Bin; Wang, Wei; Liu, Yongbei] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.

[Zou, Wei] Hunan Prov Maternal & Child Hlth Care Hosp, NHC Key Lab Birth Defects Res Prevent & Treatment, Changsha 410008, Hunan, Peoples R China.

通讯机构：

[Liu, Bin; Liu, B; Wang, W] H

Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.

Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.

语种：

英文

期刊：

Analyst

ISSN：

0003-2654

年：

2019

卷：

144

期：

页码：

1187-1196

DOI：

10.1039/c8an02147c

基金类别：

Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [h14JJ2049]; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81501218, 81673579, 81874369, 31672457]

机构署名：

本校为通讯机构

院系归属：

药学院

摘要：

T4 polynucleotide kinase (PNK) is the primary member of the 5'-kinase family that can transfer the gamma-phosphate residue of ATP to the 5'-hydroxyl group of oligonucleotides. In this article, using the differential quenching ability of reduced graphene oxide (rGO) towards the fluorophore-labeled DNA probe, we propose a novel method for detecting T4 PNK activity assisted by ligase reaction. Under the optimized conditions, the detection limit of T4 PNK was estimated to be 0.0002 U muL(-1) in the linear region of 0.001 U muL(-1)-0.1 U muL(-1). Additionally, the developed method was used to scree...

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A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction

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