期刊论文

Cao, J.L.

中文

中药材

1001-4454

2006

29

10

1038-1040

10.3321/j.issn:1001-4454.2006.10.016

国家自然科学基金资助项目（30500670）；

本校为第一机构

目的:为8种人参皂苷的含量测定建立一套方法.方法:色谱柱:ODS柱(Kromasil 250mm×4.6mm,5μm),DAD检测器;流动相:A为乙腈,B为水,梯度洗脱0～35 min 19%A,35～55 min 19%～29%A,55～75 min29%A,75～100 min 29%～40%A.流速:1 ml/min,温度:室温,检测波长:203 nm.结果:用本方法对各种皂苷的测定结果:稳定性试验RSD为0.55%～2.26%,精密度试验RSD为0.85%～1.93%,重复性试验RSD为0.97%～2.72%.结论:本法稳定性好,精确度高,重复性好,可作为测定这8种皂苷的一个较好的方法.

OBJECTIVE: To establish a method to determine the contents of 8 ginsenosides in extraction of Panax ginseng by HPLC. METHODS: The sample was analyzed on an ODS chromatogram column (Kromasil 250 mm x 4.6 mm, 5 microm), with mobile phase of acetonitrile-water (gradient elution) at flow rate 1.0 ml/min and detection at wavelength of 203 nm. RESULTS: RSD of stability, precision and recurrency was 0.55%-2.26%, 0.85%-1.93% and 0.97%-2.72% respectively. CONCLUSION: This meth...