期刊论文

Dai, B.

中文

中药材

1001-4454

2010

33

12

1904-1908

10.13863/j.issn1001-4454.2010.12.028

湖南省科技厅资助项目（2008FJ3011） 湖南省科技厅资助项目（2010FJ4091） 湖南省中医药科研基金（2008099）

本校为第一机构

目的:探讨六味地黄丸中马钱苷,芍药苷,莫诺苷,丹皮酚4种入血成分组合对大鼠前脂肪细胞增殖,分化的影响,并优选其最佳浓度配伍.方法:原代培养大鼠的前脂肪细胞,将马钱苷等4种入血成分按四因素三水平的正交试验表L9(34)安排实验,同时设立空白对照组,以四甲基偶氮唑盐(MTT)法测定细胞增殖的速度,以油红O染色方法检测其对大鼠前脂肪细胞分化过程中的细胞内脂肪积聚的影响,以光密度为指标比较各因素,水平之间的差异.结果:六味地黄丸4种入血成分组合对大鼠前脂肪细胞增殖,分化的影响以组合A3B1C3D2的作用最为显著(P

OBJECTIVE: To investigate the effects of four original constituents (Loganin, Morroniside, Peoniflorin, Paeonol) of Liu-wei Di-huang pills on the proliferation and differentiation of rat preadipocyte, and optimize the best concentration by the orthogonal test. METHODS: The rat preadipocytes were cultured, then above four original constituents according to 4 factors and 3 levels to make L9 (3(4)) orthogonal test, and the control was performed at the same time. The proliferation of preadipocytes was determined by MTT method, and the accumulation of cellular lipid was determined by Oil Red O stai...