期刊论文

中文

中药材

1001-4454

2013

36

12

1917-1921

10.13863/j.issn1001-4454.2013.12.016

国家自然科学基金青年基金（30901971,81270055,81073142） 湖南省教育厅“十二五”中药学重点学科（湘教通[2011]76号）

本校为第一机构

目的:揭示同一GAP产地同一批次的鲜蕺菜植株间的蛋白质多样性,为蕺菜“节点代谢网络”的构建奠定基础。方法:采用3种方法从3个角度对蕺菜植株间的蛋白质进行多样性研究,即采用Ramagli改进的Bradford法、SDS-PAGE凝胶电泳法和双波长薄层扫描法分别研究其总蛋白含量多样性、蛋白种类差异性及各种类的含量变异性。结果:53株蕺菜蛋白条带分子量大多集中在6.5~97.2 kDa范围内,蛋白种类差异性不明显,主要表现为条带颜色深浅不一;蛋白电泳条带扫描图的总量统计矩零阶矩(AUC_T)、一阶矩(MCRT_T)和二阶矩(VCRT_T)的RSD分别为40.92%、6.01%和18.57%。结论:同一GAP产地不同植株鲜蕺菜个体间蛋白质具有丰富的...

OBJECTIVE: To reveal protein diversity between the same batch of fresh Houttuynia cordata in the same GAP base,and to lay the foundation construction for "node metabolic network". METHODS: Three methods including the Ramagli improved Bradford law, SDS-PAGE gel electrophoresis method and double wavelength thin-layer scanning method were used to study the total protein content diversity, protein species diversity and various kinds of content variability. RESULTS: The molecular weight of 53 plant protein mostly concentrated in the range of 6.5-97.2 kDa, the species diversity was not obvious with ...