期刊论文

中文

中药材

1001-4454

2014

37

8

1463-1466

10.13863/j.issn1001-4454.2014.08.039

科技部“重大新药创制”（2013ZX09201019） 教育部高等学校博士学科点专项科研基金（20124323120004） 湖南省自然科学基金（13JJ4089） 湖湘青年科技创新创业平台人才项目（2013）

本校为第一机构

目的:建立不同批次夏桑菊颗粒的指纹图谱。方法:采用UPLC,建立12批夏桑菊颗粒的指纹图谱。ACQUITY UPLC BEH C_(18)色谱柱(2.1 mm×50 mm,1.7 μm),流动相为乙腈-0.5%醋酸溶液,梯度洗脱,柱温30℃,流速0.4 mL/min,检测波长320 nm,标定了16个色谱峰,分别采用相似度软件、聚类分析和主成分分析等方法,对12批样品进行系统的比较与归类。结果:建立了夏桑菊颗粒专属性的UPLC指纹图谱,将不同批次的样品分为6大类。结论:该方法重复性好,简便可靠,可用于夏桑菊颗粒的快速鉴别,可为夏桑菊颗粒的质量控制提供依据。

OBJECTIVE: To establish a UPLC fingerprint method of Xiasangju Granules. METHODS: UPLC analysis was performed on a Waters ACQUITY UPLC H-Class system and carried out at 30 degrees C on a Waters Column ACQUITY UPLC BEH C18 (2.1 mm x 50 mm, 1.7 mum). A binary gradient elution system was composed of acetonitrile (phase A)and 0.5% acetic acid solution (phase B). Detection was performed at the wavelength of 320 nm,the mobile flow rate was at 0.4 mL/min. A matrix including 16 variations (characteristic peaks area)and 12 samples was constructed for similarity evaluation, cluster analysis and principl...