期刊论文

中文

湖南中医药大学学报

1674-070X

2016

36

10

18-21

10.3969/j.issn.1674-070X.2016.10.005

81373508:国家自然科学基金 14JJ2113:湖南省自然科学基金 16K063:教育厅项目 201606:湖南省中医药科研项目 :中西医结合基础湖南省重点学科建设项目

本校为第一机构

目的探讨急性缺血性中风（AIS）瘀毒病机的分子机制及解毒化瘀方对凝血酶合并缺氧损伤的保护作用。方法（1）用凝血酶合并缺氧损伤PC12细胞,模拟急性缺血性中风的体外细胞模型。（2）将细胞分为空白组,模型组,解毒化瘀方含药血浆组和PD98059组（MEK抑制剂组）,应用荧光定量实时RT-PCR方法检测MEK1、PAR-1及ERK1/2的m RNA表达,应用免疫荧光法检测ERK1/2、P-ERK1/2的蛋白表达。结果 PCR结果提示:模型组MEK1、PAR-1及ERK1/2的m RNA表达与空白对照组相比显著升高（P

Objective To investigate the molecular mechanism of cerebral vascular occlusion and the mechanism of brain cell damage induced by acute ischemic stroke (AIS) and the protective effect of Jiedu Huayu Fang. Methods (1) PC12 cells were used to establish acute ischemic stroke in vitro. (2) The cells were divided into blank group, model group, Jiedu Huayu Fang serum group and PD98059 group (MEK inhibitor group). The mRNA expression of MEK1, PAR-1 and ERK1/2 was detected by Real-time PCR, and the protein expression of P-ERK1/2 and ERK1/2 was detected by immunofluorescence assay. Results PCR results ...