After successively introducing elements of a triple-helix aptamer probe (TAP) and a luminol–hydrogen peroxide (H2O2) solution, we proposed a brand-new chemiluminescence (CL)-based biosensor for the assay of ochratoxin A (OTA). Primarily, the TAP contains an aptamer loop, two DNA segment stems, and a triplex-forming oligonucleotide (signal probe). In the presence of a target, the aptamer sequence binds to the target with an impressive affinity. This association leads to a conformational change in the TAP, thus releasing the signal probe. Sequen...
