期刊论文

Effects of six ingredient rehmannia decoction on expression of HIF-1α and PAI-1 in chronic renal failure rats

中文

中国现代医生

1673-9701

2014

52

36

9-12

2013118:湖南省中医药科研项目 2013SK3085:湖南省科技厅科学研究项目

本校为第一机构

目的 探讨六味地黄汤抗肾间质纤维化的作用机制.方法 5/6肾切除法制备慢性肾衰模型,分假手术组、模型组、六味地黄汤组和依那普利组.六味地黄汤组予以6.25 g/(kg·d)六味地黄汤灌胃,依那普利组予以10 mg/(kg·d)依那普利灌胃,假手术组和模型组予以10 mL/(kg·d)蒸馏水灌胃,每天1次,连续灌胃12周.造模12周后,应用免疫组化法和RT-PCR法分别检测HIF-1α、PAI-1在肾组织中蛋白水平和基因表达.结果 与假手术组比较,模型组大鼠肾组织中HIF-1α、PAI-1蛋白水平和基因表达均明显升高(P＜0.05);与模型组比较,六味地黄汤组和依那普利组大鼠肾组织中HIF-1α、PAI-1蛋白水平和基因表达均明显降低(P＜0.05),且...

Objective To investigate the mechanism of six ingredient rehmannia decoction against renal interstitial fibrosis. Methods The rat model of renal interstitial fibrosis were induced by 5/6 nephrectomy, rats were divided into sham operation group, model group, six ingredient rehmannia decoction group and enalapril group. Six ingredient rehmannia decoction group was given 6.25 g/（kg· d） of six ingredient rehmannia decoction gavage, enalapril group was given 10 mg/（kg·d） enalapril lavage, sham operation group and model group were given 10 mL/（...