期刊论文

Yan, J.

中文

结合医学学报：英文版

2095-4964

2007

5

3

338-342

10.3736/jcim20070321

教育部高等学校博士学科点专项科研基金资助项目（No.20050541003） 湖南省教育厅资助项目（No.05C364）

本校为第一且通讯机构

目的：探讨电针大鼠胃经穴后提取的血清对胃黏膜细胞表皮生长因子受体（epidermal growth factor receptor，EGFR）后信息物质磷脂酶Cγ-1（phospholipase Cγ-1，PLCγ-1）、蛋白激酶C（protein kinase C，PKC）和c-myc表达的影响。 方法：60只大鼠随机分为正常组、胃经组、胆经组、胃经＋PD153035组和胆经＋PD153035组，采用水浸束缚法制作胃黏膜损伤大鼠模型，链霉蛋白酶消化法分离胃黏膜细胞，分别用EGFR抑制剂PD153035和血清孵育胃黏膜细胞，应用酶联免疫吸附法检测PLCγ-1活性，同位素掺入法检测PKC活性，逆转录聚合酶链反应法检测c-myc的表达水平。 结果：正常组大鼠胃黏膜细胞PLCγ-1、...

OBJECTIVE: To study the effects of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expressions of epidermal growth factor receptor (EGFR) signaling substances phospholipase C gamma-1 (PLC gamma-1), protein kinase C (PKC) and c-myc in gastric mucosal cells. METHODS: Sixty rats were randomly divided into normal group, stomach meridian group, gallbladder meridian group, stomach meridian plus PD153035 group and gallbladder meridian plus PD153035 group. Water-immersion and restrained stress methods were adopted for inducing gastric mucosal injury in the ...