期刊论文

Adjuvant effect of Curculigo Orchioides Polysaccharide on Immunogenicity and Protection of Influenza Vaccine

中文

中药药理与临床

1001-859X

2020

36

05

84-87

10.13412/j.cnki.zyyl.2020.05.004

国家自然科学基金（编号:81774126）； 贵州省科学技术基金项目（编号:黔科合基础[2017]1009）； 贵州省科技创新人才团队（编号:黔科合平台人才[2020]5010）；

本校为其他机构

目的:研究仙茅多糖辅助流感裂解疫苗对小鼠抵抗流感病毒感染的免疫效应。方法:将健康昆明小鼠140只分为空白对照组、模型对照组、仙茅多糖对照组、疫苗对照组以及仙茅多糖不同剂量组。疫苗对照组以及仙茅多糖不同剂量组于第0、14 d采用流感裂解疫苗皮下注射免疫,仙茅多糖不同剂量组在两次疫苗免疫之前分别使用10、20、40 mg/kg的仙茅多糖连续肌肉注射3 d。第21 d检测各组小鼠血清中特异性IgG、IgG1和IgG2a的含量,同时用流感病毒液20 μL/只滴鼻感染小鼠,观察小鼠一般情况及21 d内自然死亡情况,并检测肺脏病毒滴度及病理学变化。结果:与空白对照组相比,模型对照组小鼠有显著流感症状,11 d死亡率1...

Objective: To observe the immune effect of curculigo orchioides polysaccharide(COP) assisted influenza vaccine against influenza virus infection in mice. Mothods: 140 healthy Kunming mice were divided into the control group, the model group,COP group, vaccine group and different doses of COP groups. Mice in vaccine group and different doses of COP groups were subcutaneously immunized with influenza vaccine and immunized again on the 14th day after the first immunization. Simultaneously,COP (40, 20, 10 mg/kg) were intramuscularly injected for 3 ...