Construction of COX-2 short hairpin RNA expression vector and its inhibitory effect on hepatic fibrosis

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Xie, Ni;Liao, Hong Wu;Ou, Wen Sheng;Zhou, Xu;Hu, Yang;...

通讯作者：

Yang, XF;Liao, DF

作者机构：

[Yang, Xue Feng; Fu, Nian; Zhou, Xu; Yang, XF; Xie, Ni; Liao, Hong Wu; Hu, Yang] Univ South China, Affiliated Nanhua Hosp, Dept Gastroenterol, Hengyang, Hunan, Peoples R China.

[Liao, DF; Liao, Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Changsha, Hunan, Peoples R China.

[Ou, Wen Sheng] First Peoples Hosp Chenzhou, Dept Gastroenterol, Chenzhou, Hunan, Peoples R China.

通讯机构：

[Liao, DF ] H

[Yang, XF ] U

Univ South China, Affiliated Nanhua Hosp, Dept Gastroenterol, Hengyang, Hunan, Peoples R China.

Hunan Univ Chinese Med, Sch Pharm, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Changsha, Hunan, Peoples R China.

语种：

英文

关键词：

Cyclooxygenase-2;liver fibrosis;recombinant adenovirus vector;short hairpin RNA

期刊：

Biotechnology & Biotechnological Equipment

ISSN：

1310-2818

年：

2018

卷：

期：

页码：

653-662

DOI：

10.1080/13102818.2018.1431569

基金类别：

This work was supported by the National Natural Science Foundation of China [grant number 81373465] and the Hunan Provincial Health and Family Planning Commission Research Program [grant number A2017015].

机构署名：

本校为通讯机构

院系归属：

药学院

摘要：

The aim of this study was to construct recombinant adenovirus vector carrying a short hairpin RNA (shRNA) that can exclusively target cyclooxygenase-2 (COX-2) gene expression and observe its inhibitory effect on hepatic fibrosis. We designed and synthesized three oligonucleotide sequences, and cloned those into a shuttle vector, pYr-1.1-hU6-EGFP, after annealing. The restriction-enzyme digestion and sequencing analyses confirmed that the constructed recombinant eukaryotic expression vector was correct. A recombination reaction using LR Clonase ...

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Construction of COX-2 short hairpin RNA expression vector and its inhibitory effect on hepatic fibrosis

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