期刊论文

中文

亚太传统医药

1673-2197

2019

15

5

77-81

10.11954/ytctyy.201905024

湖南省科技厅重点项目（2016SK2034）；

本校为第一机构

目的:建立五汁饮中阿魏酸和多糖的含量测定方法并进行方法学考察,以便控制该制剂的质量。方法:五汁饮中阿魏酸定量分析采用HPLC法,使用Ultimate 3000色谱柱,固定相为碳十八键合硅胶,流动相为甲醇-0.5%乙酸水溶液（30∶70）,流速1mL·min-1,运行时间30min,检测波长321nm,柱温30℃;五汁饮中多糖定量分析采用苯酚-硫酸比色法。结果:五汁饮中阿魏酸含量测定结果表明,阴性无干扰,仪器精密度RSD为0.96%,供试品溶液48h内稳定（RSD为1.31%）,重复性试验RSD为1.14%（n=6）,平均加样回收率（n=9）98.69%,RSD为1.0%。阿魏酸理论塔板数为4 000,分离度为1.65。五汁饮中多糖平均含量为63.75%（RSD=5.2%,n=6）,...

Objective:To establish the determination method of ferulic acid and polysaccharide content in the Five Juices Decoction and to carry out the methodology,so as to control the quality of preparation.Methods:The quantitative analysis of ferulic acid in the Five Juices Decoction was carried out by using the Ultimate 3000chromatographic column.The stationary phase was carbon octade-the running time was 30min.The detection wavelength was 321nm;the column temperature was 30℃;the quantitative analysis of poly-content in the Five Juices Decoction showe...