期刊论文

Sun, J.

中文

中草药

0253-2670

2013

44

10

1275-1279

10.7501/j.issn.0253-2670.2013.10.013

陕西省科学技术发展计划项目（2012SF2-07） 陕西省教育厅中药制药省级重点建设学科资助项目（100800）

本校为其他机构

目的优选大孔吸附树脂纯化陕产黄花油点草总黄酮部位的最佳工艺。方法以紫外分光光度法测定总黄酮的量。采用每克树脂纯化转移总黄酮的量为指标,通过考察动态吸附-解吸附、静态吸附-解吸附过程,对AB-8、D-101、HPD-450、HPD-600、HPD-700大孔树脂进行了优选;再进一步考察提取药液质量浓度、洗脱剂用量、径高比、药液的pH值对树脂纯化工艺的影响,确定最佳工艺条件,并对优选工艺条件进行了验证试验。结果药液质量浓度为1.2 g/mL,径高比为1∶5,药液pH 6.47,生药材-树脂为1∶3,上样体积流量为1 BV/h,洗脱液为40%乙醇,洗脱体积流量为1 BV/h,洗脱体积为2 BV。结论该工艺科学合理,可有效实现总黄酮部位...

Objective: To optimize the purification technology of total flavonoids in Tricyrtis maculata (TFTM) produced in Shaanxi province using macroporous adsorption resin. Methods: The content of TFTM was determined with ultraviolet spectrophotometry. Taking the content of total flavonoids purified and transferred by per gram of resin as the indicator, the processes of dynamic adsorption-desorption and static adsorption-desorption were observed, and five macroporous resins, AB-8, D-101, HPD-450, HPD-600, and HPD-700 were optimized. The parallel invastigations were carried out to study the effects of ...