期刊论文

Cloning of MADS-box gene AGL15 from Lonicera macranthoides and analysis on its bioinformatics and expression

Tong, Q.-Z.

中文

中草药

0253-2670

2016

47

4

640-647

10.7501/j.issn.0253-2670.2016.04.019

国家自然科学基金资助项目(81203007)； 湖南省自然科学基金资助(13JJ9010)； 湖南省教育厅科技创新平台(14K071)； 湖南省研究生科研创新项目(CX2014B368)； 湖南省“中药学”重点学科建设项目资助(湘教通[2011]76号:zy201403,zy201505)；

本校为第一且通讯机构

目的 基于前期对灰毡毛忍冬Lonicera macranthoides转录组测序结果,从灰毡毛忍冬RNA中克隆灰毡毛忍冬MADS-box基因家族蕾期延长相关基因AGL15,并对其进行生物信息学及表达分析.方法 对灰毡毛忍冬总RNA进行反转录酶链式反应(reverse transcription-PCR,RT-PCR)和cDNA末端快速扩增反应(rapid amplification of cDNA ends,RACE),获得完整的开放阅读框(ORF).运用生物信息学的方法对该序列进行同源性分析和相似性比较,预测编码蛋白,并对其进行各种理化性质分析.运用半定量PCR测得该基因在灰毡毛忍冬不同部位的表达情况.结果 获得AGL15基因,ORF长795 bp,编码264个氨基酸,与葡萄Vitis vinifera中MADS-b...

Objective: To clone the MADS-box gene denoted AGL15 from total RNA of Lonicera macranthoides based on previous transcriptome sequencing data and analyze the bioinformatics and expression of the gene. Methods: The gene containing intact open reading frame (ORF) was cloned by reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The similarity comparison and homology analysis on the sequence were carried out using bioinformatic method, the coding protein was predicted and the physicochemical properties were analyzed. The...