期刊论文

Purification of total flavonoids from leaves of Acanthopanax henryi

Liu, X.-Q.

中文

中草药

0253-2670

2017

48

22

4661-4667

10.7501/j.issn.0253-2670.2017.22.011

湖南省自然科学基金重点资助项目（11JJ2042） 湖南省中药学“十二五”重点学科建设项目 湖南中医药大学药物分析学“十二五”校级重点学科建设项目 长沙市科技计划项目（kq1701119）

本校为第一且通讯机构

目的研究优化糙叶五加叶总黄酮的大孔吸附树脂纯化工艺。方法以处理后的总黄酮的质量分数和总黄酮得率为考察指标,采用单因素实验结合响应曲面设计法进行纯化工艺优化研究。结果D101型大孔吸附树脂对糙叶五加叶总黄酮有较好的吸附和洗脱效果,其最佳的纯化工艺条件为色谱柱的径高比为1∶10,25.0 g D101大孔树脂的上样量为750 mg,溶剂体系为50%乙醇,洗脱体积流量为5 mL/min,洗脱体积为130 mL。在此最佳优化条件下,糙叶五加叶总黄酮的理论质量分数为75.69%,实际质量分数达到75.87%;总黄酮得率为30.13%。结论 D101型大孔吸附树脂宜于纯化糙叶五加叶中的总黄酮,且响应面法能有效地优化其纯化工艺。

Objective To optimize the purification technology of total flavonoids from the leaves of Acanthopanax henryi by macroporous resin. Methods Using the purity and yield of total flavonoids as indexes, the single factor experiment combined with response surface methodology (RSM) was used to optimize the purification technology. Results It showed that D101 macroporous resin had good adsorption and desorption effects. The optimal purification conditions were as follows: diameter height ratio was 1:10, the loading amount was 750 mg each 25 g D101 macr...