To study the chemical constituents of Achyranthes aspera. Methods: The constituents were separated and purified by silica gel, ODS column chromatography, and recrystallization, and their structures were identified on the basis of physicochemical properties and spectral data. Results: Nine compounds were isolated and identified as 5,2’-dimethoxy-6- (methoxymethyl)-7-hydroxy-isoflavonol (1), oleanolic acid (2), oleanolic acid 28-O-β-D-glucopyranosyl ester (3), codonolactone (4), 3-formylindole (5), indole-3-carboxylic acid (6), 4-[2-formyl-5-(m...