期刊论文

Optimization of prescription process of cantharidin nanostructured lipid carrier by central composite design-response surface methodology

Xia, X.-H.

中文

中草药

0253-2670

2019

50

17

4114-4122

10.7501/j.issn.0253-2670.2019.17.016

湖南省重点研发计划项目(2017SK2122) 国家自然科学基金面上项目(81573621) 湖南省研究生科研创新项目(CX20190574).

本校为第一机构

目的 将斑蝥毒性成分斑蝥素(CTD)整合在新型纳米载体(纳米结构脂质载体,NLC)中,并优化斑蝥素纳米结构脂质载体(CTD-NLC)处方工艺,从而降低斑蝥素的毒性并增强其靶向性.方法 乳化超声分散法制备CTD-NLC,建立透析法测定其包封率,以平均粒径、粒径分布(多分散指数,PDI)、Zeta电位、包封率与载药量为考察指标,采用单因素考察与星点设计-效应面法(CCD-RSM)优化CTD-NLC的处方工艺,多元二次项拟合评价指标与因素间的模型方程,对拟合方程进行方差分析,效应面法预测最优处方.结果 优化后的CTD-NLC处方工艺:脂质总量为453.66 mg、固体脂质与液体脂质的比例为1:2、总稳定剂质量浓度为16.9 mg/mL、Pluronic ...

Objective: To integrate the toxic component of cantharidin (CTD) into a novel nanostructured lipid carrier (NLC) and optimize the cantharidin nanostructured lipid carrier (CTD-NLC) formulation process to reduce the toxicity of CTD and enhance its targeting. Methods: CTD-NLC was prepared by emulsified ultrasonic dispersion method. The encapsulation efficiency was determined by dialysis method. The average particle size, particle size distribution (polydispersity index, PDI), Zeta potential, encapsulation efficiency, and drug loading were taken a...