期刊论文

HPLC fingerprint of Xintongtai Granules

Li, Y.

中文

中草药

0253-2670

2019

50

22

5478-5483

10.7501/j.issn.0253-2670.2019.22.014

国家自然科学基金资助项目(81673955) 湖南省自然科学基金资助项目(2017JJ2206) 湖南中医药大学中药学一流学科建设项目(4901-0200002006) 湖南省大学生创新创业训练计划项目(S201910541009)。

本校为第一且通讯机构

目的 建立心痛泰颗粒的HPLC指纹图谱,为其质量评价提供依据.方法 采用 Phenomenext Luna C18柱(250 mm×4.6mm,5μm)色谱柱,以0.1％甲酸溶液-甲醇为流动相,检测波长为280nm,体积流量为1.0mL/min; 柱温为 30℃.测定 10批心痛泰颗粒样品,应用中药色谱指纹图谱相似度评价系统(2012版)建立心痛泰颗粒指纹图谱共有模式图,并计算相似度,再通过对照晶色谱图对共有峰进行指认.结果 通过 10批样品的测定,建立心痛泰颗粒HPLC指纹图谱,相似度均在 0.95以上,共标定 26个共有色谱峰,共有峰全部归属到各药材,其中 3个共有峰(21、22、26号峰)为丹参专属,8个共有峰(4～10、16号峰)为葛根专属,7个共有峰(13、15、...

Objective: To establish an HPLC method for fingerprint analysis of Xintongtai Granules (XG) for its quality control. Methods: The chromatographic behaviors were obtained by a Phenomenext Luna C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution using 0.1% formic acid solution-methanol as the mobile phase. The detection wavelength was 280 nm, the volume flow rate was 1.0 mL/min, and the column temperature was 30 ℃. The samples of 10 batches of XG were determined, and the chromatographic fingerprint similarity evaluation system of Chinese ...