期刊论文

HPLC fingerprint of Zhixue Tiaojing Granule and determination of main components

Wang, W.

中文

中草药

0253-2670

2019

50

15

3642-3647

10.7501/j.issn.0253-2670.2019.15.019

长沙市2018年度第二批事前立项事后补助科技计划项目（重大专项）:止血调经颗粒关键技术研究及产业化（kh1802009）；

本校为第一且通讯机构

目的首次建立止血调经颗粒甲醇提取物的HPLC指纹图谱,同时对其中芍药苷和毛蕊异黄酮葡萄糖苷的含量进行测定。方法赛默飞UltimateTM U3000系列高效液相色谱仪;Thermo Acclaim~(TM) 120 C_(18)色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈-水,梯度洗脱;体积流量为1 mL/min;柱温30 ℃;检测波长254 nm;进样量10 μL。结果止血调经颗粒甲醇提取物指纹图谱共标记了11个共有峰,10批样品的相似度均大于0.95;其中,指纹峰分别来自黄芪(1、5、7、10号峰)、党参(1号峰)、续断(2、3号峰)、白芍(4号峰)、当归(6号峰)、茜草(6、8、11号峰)和仙鹤草(9号峰),确定3个已知成分,分别为芍药苷、毛蕊异黄酮葡萄糖苷和芒...

Objective: To establish an HPLC fingerprint of methanol extract of Zhixue Tiaojing Granule (ZTG), and determine the content of paeoniflorin and calycosin-7-glucosid. Methods: HPLC analysis was performed on an Thermo UltiMateTM U3000 LC system and carried out at 30 ℃ on a column of Thermo AcclaimTM 120 C18 (250 mm × 4.6 mm, 5 μm). Gradient elution system was composed of water solution (phase A) and acetonitrile (phase B). Detection was performed at the wavelength of 254 nm, the mobile flow rate was 1 mL/min, the column temperature was 30 ℃, ...