期刊论文

Effect of borneol combined with astragaloside IV and Panax notoginseng saponins on transporter proteins of blood-brain barrier after cerebral ischemia-reperfusion

Huang, X.-P.

中文

中草药

0253-2670

2019

50

18

4389-4397

10.7501/j.issn.0253-2670.2019.18.019

湖南中医药大学“十三五”一级学科基础医学建设项目(No:06) 国家自然科学基金资助项目(81573875) 湖南省科技厅科技创新平台与人才计划-中医脑病临床研究中心(2017SK4005) 2017年度湖南省大学生研究性学习和创新性实验计划项目(1029) “中医药防治心脑血管疾病基础研究”湖南省自然科学创新群体基金.

本校为第一机构

目的从血脑屏障(blood-brain barrier,BBB)转运蛋白角度探讨冰片配伍黄芪甲苷(astragalosides IV,AST IV)和三七总皂苷(Panax notoginseng saponins,PNS)在脑缺血再灌注状态下促进药物成分入脑的作用。方法采用大鼠局灶性脑缺血再灌注模型,ig给予冰片、AST IV、PNS及其配伍药物,2,3,5-氯化三苯四氮唑(2,3,5-triphenyl tetrazolium chloride, TTC)染色法测定脑组织损伤,Western blotting法检测脑组织外排蛋白P-糖蛋白(P-glycoprotein,P-gp)和多药耐药相关蛋白1(multidrug resistance protein-1,MRP-1)、MRP-2、MRP-4、MRP-5及摄取蛋白有机阳离子转运体3(organic cation transporter 3,OCT3)、有机...

Objective: To investigate the effects of borneol combined with astragaloside IV (AST IV) and Panax notoginseng saponins (PNS) on promoting the active components into the brain and anti-brain injury through the regulation of transporter proteins of blood-brain barrier (BBB) in the state of cerebral ischemia-reperfusion. Methods: Focal cerebral ischemia-reperfusion model in rats was established, borneol, AST IV, PNS and the combination were administered by gavage, brain infarction rate was evaluated by 2,3,5-triphenyl tetrazolium chloride (TTC) s...