期刊论文

Establishment and application of chemical fingerprint and pattern recognition for Panacis Japonici Rhizoma

Yan, J.-Y.

中文

中草药

0253-2670

2019

50

1

217-224

10.7501/j.issn.0253-2670.2019.01.032

湖南省中医药管理局重点项目(201811号) 湖南中医药大学-层次学科中药学资助(湘教通[2011]76号).

本校为第一且通讯机构

目的建立并识别不同产地白三七药材HPLC指纹图谱,为其质量控制及标准制定提供参考。方法采用HPLC法建立白三七指纹图谱,同时应用相似度评价、聚类分析和主成分分析(PCA)对实验数据进行识别,以分析不同产地白三七药材间及同科同属5种药材的相似性及差异性,并通过总量统计矩法对白三七药材共有峰进行定性定量分析。结果白三七指纹图谱共标定16个共有峰,并指认了人参皂苷Rg 1、Re、Rb 1及竹节参皂苷V、IV、IVa色谱峰;其中竹节参皂苷V和IV在不同批次白三七样品中的质量波动较小;各产地白三七样品除产自四川大凉山样品(S7)外相似度在0.90以上;聚类分析及主成分分析均将白三七样品分为3类;五加科人参属...

Objective To establish and identify HPLC fingerprints of Panacis Japonici Rhizoma from different regions, and provide a scientific method for quality its control and standardization. Methods The fingerprints of Panacis Japonici Rhizoma were established based on HPLC method. The similarity evaluation, cluster analysis (CA), and principal component analysis (PCA) were applied for the experimental data, in order to find out the similarities and differences among the 10 batches of Panacis Japonici Rhizoma from different regions. Meanwhile, the qual...