期刊论文

Effects of astragaloside IV combined with Panax notoginseng saponins on proliferation,apoptosis,migration and neuronal differentiation of bone marrow mesenchymal stem cells in OGD/R model rats

Deng, C.-Q.

中文

中草药

0253-2670

2019

50

23

5795-5801

10.7501/j.issn.0253-2670.2019.23.022

湖南省自然科学基金项目(2018JJ3382) 湖南省教育厅优秀青年项目(18B236) 湖南省科技厅科技创新平台与人才计划——中医脑病临床研究中心(2017SK4005) 湖南省自然科学创新群体基金:中医药防治心脑血管疾病基础研究。

本校为第一且通讯机构

目的观察黄芪甲苷(AST IV)配伍三七总皂苷(PNS)对氧糖剥夺后再复糖复氧(OGD/R)大鼠骨髓间充质干细胞(BMSCs)增殖、凋亡、迁移及神经分化的影响。方法全骨髓贴壁法分离、培养、扩增、纯化BMSCs,流式细胞仪检测BMSCs表面标志物CD29、CD90、CD34、CD45阳性表达率。取第3代BMSCs,采用AST IV与PNS高(100 μmol/L+60 μmol/L)、中(50 μmol/L+30 μmol/L)、低(25 μmol/L+15 μmol/L)剂量配伍预处理24 h,以OGD/R建立缺血再灌注损伤模型,同时设立对照组和模型组。CCK-8法测定细胞增殖情况,流式细胞术检测细胞凋亡,Transwell实验检测细胞迁移,巢蛋白(Nestin)/神经元特异性烯醇化酶(NSE)、Nestin/胶质纤维...

Objective: To observe the effects of astragaloside IV (AST IV) combined with Panax Notoginseng saponins (PNS) on proliferation, apoptosis, migration and neuronal differentiation of oxygen glucosedeprivation/reoxygenation model rat bone marrow mesenchymal stem cells (BMSCs). Methods: BMSCs were isolated, cultured, amplified and purified by the whole bone marrow adherent method. The positive expression rates of BMSCs surface markers, CD29, CD90, CD34, and CD45 were detected by flow cytometry. The third generation of BMSCs was pretreated with AST ...