期刊论文

Optimization of purification of total polyphenols from Acanthopanan trifoliates leaves by macroporous resin

Wang, H.-L.

中文

中草药

0253-2670

2019

50

13

3071-3076

10.7501/j.issn.0253-2670.2019.13.012

湖南省自然科学基金项目(2019JJ40223) 浙江理工大学2018年本科生科研创新计划项目.

本校为其他机构

目的 考察大孔吸附树脂对白筋叶总多酚的吸附性能和纯化效果,寻找白筋叶总多酚纯化的较优工艺.方法 采用Folin-Ciocalteu比色法定量,比较5种不同型号大孔吸附树脂对药食同源植物白簕叶中总多酚的吸附和解吸效果,从中筛选出适合分离纯化白簕叶总多酚的树脂,并对其吸附和解吸条件进行考察和优化.经过单因素实验,确定最终优化工艺.结果 所得优化工艺为以质量浓度为1.0 mg/mL,pH值为3.0左右的白簕叶总多酚粗提液作为上样液,同时控制其体积流量为2.0mL/min,上样量控制为30 mL;然后以体积分数50％的乙醇水溶液洗脱,调节pH值为6.0,并控制洗脱体积流量为2.0 mL/min,洗脱量为40 mL.经HPD100型树脂纯化后,...

Objective: To investigate the adsorption performance and purification effect of macroporous adsorption resin on total polyphenols in Acanthopanan trifoliates leaves, and to determine the technological conditions for the purification of total polyphenols from A. trifoliates leaves. Methods: The Folin-Ciocalteu colorimetric method was used to quantify the adsorption and desorption effects of five macroporous adsorption resins on the total polyphenols in the A. trifoliates leaves. The resin suitable for separation and purification of total polyphe...