期刊论文

Determination of seven saponins components in Panacis Japonici Rhizoma with quantitative analysis of multi-components by single marker

Yan, J.-Y.

中文

中草药

0253-2670

2019

50

17

4164-4169

10.7501/j.issn.0253-2670.2019.17.023

湖南省重点研发计划项目(2017SK2123) 湖南省中医药科研计划重点项目(201811) 长沙市科技计划重点项目(kq1801034) 湖南中医药大学一层次学科中药学资助(湘教通[2011]76).

本校为第一机构

目的建立一测多评法同时测定竹节参中7种皂苷类成分的含量,并验证此方法在竹节参中应用的可行性与适用性。方法采用HPLC法,以竹节参皂苷Ⅴ为内参物,建立人参皂苷Rg_1、Re、Rb_1,假人参皂苷RT_1,竹节参皂苷Ⅳ、Ⅳa的相对校正因子(f_(s/i)),并利用f_(s/i)计算竹节参样品中成分的含量,同时用外标法测定各成分的含量,比较2种方法的差异。结果人参皂苷Rg_1、Re、Rb_1,假人参皂苷RT_1,竹节参皂苷Ⅳ和Ⅳa的f_(s/i)分别为1.286 7、1.432 7、0.966 6、0.962 4、1.207 2、 0.938 4。采用一测多评法与外标法得到的10批竹节参样品各成分含量无显著性差异。结论建立的一测多评法准确性高,可用于竹节参皂苷类成...

Objective: To establish quantitative analysis of multi-components by single marker (QAMS) method for simultaneous determining the content of seven saponins in Panacis Japonici Rhizoma, and to evaluate the adaptation and application of QAMS method in the quality control of Panacis Japonici Rhizoma. Methods: The relative factor (fs/i) of ginsenoside Rg1, Re, Rb1, pseudoginsenoside RT1, chikusetsusaponin IV and Ⅳa were established by HPLC method with chikusetsusaponin V as internal standard, which were used to calculate the content of seven sapon...