期刊论文

Effect of influenza A virus infection on pulmonary flora and chemokines CCL5 and CXCL10 in mice and intervention of Maxing Shigan Decoction

Lu, F.-G.

中文

中草药

0253-2670

2020

51

21

5523-5537

10.7501/j.issn.0253-2670.2020.21.017

国家自然科学基金资助项目(81774126) 国家自然科学基金资助项目(82074250) 湖南省自然科学基金项目(2020JJ4063) 湖南中医药大学校级研究生培养质量工程项目(2019CX05) 湖南中医药大学校级研究生培养质量工程项目(2019CX22) 湖南中医药大学中西医结合一流学科开放基金项目(2018ZXYJH11) 湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目(NO:15) 湖南中医药大学一流学科《基础医学》资助项目(NO:1) 贵州省科技创新人才团队(黔科合平台人才[2020]5010)。

本校为第一且通讯机构

目的探索麻杏石甘汤(Maxing Shigan Decoction,MSD)通过影响小鼠肺部菌群及趋化因子CCL5和CXCL10表达而防治流感病毒感染的潜在机制。方法通过鼻腔接种法建立A型流感病毒感染小鼠模型,经ig给药或生理盐水3、7 d后,计算肺指数和肺指数抑制率,HE染色检测肺组织病理变化,应用免疫组化、酶联免疫吸附试验(ELISA)、实时荧光定量PCR(RT-PCR)法检测肺组织中CCL5和CXCL10蛋白和mRNA表达变化,并取小鼠肺组织进行16S rRNA基因V3~ V4可变区测序、物种注释及聚类,分析Alpha多样性和Beta多样性及组间差异物种,分析CCL5、CXCL10表达与肺部菌群变化的关系。结果给药3 d后,模型组肺指数明显高于对照组(P

Objective: To investigate the potential mechanism of Maxing Shigan Decoction (MSD) in the prevention and treatment of influenza virus infection by influencing pulmonary flora and expression of chemokines CCL5 and CXCL10 of mice. Method The infected mice model of influenza A virus was tested by intranasal inoculation. After 3 and 7 d of gavage or saline, the lung index and lung index inhibition rate were calculated. Pathological changes of lung tissue were detected by HE staining. The expression of CCL5 and CXCL10 in the lung tissue of mice was ...