期刊论文

Researches on molecular mechanism of Dihuang Yinzi Decoction for application to Alzheimer's disease from regulation of apoptotic network

You, B.-W.

中文

中草药

0253-2670

2020

51

21

5548-5558

10.7501/j.issn.0253-2670.2020.21.019

国家自然科学基金面上项目(81774129) 国家自然科学基金青年项目(82004184) 湖南省自然科学基金项目(2020JJ8023) 湖南省中医药管理局科研项目(2018025,201609) 湖南省教育厅科研项目(15K088,18B246)。

本校为第一机构

目的利用中药网络药理学和分子生物学从凋亡网络的调控研究地黄饮子(DHYZ)治疗阿尔兹海默病(AD)的分子机制。方法利用网络药理学方法筛选和预测DHYZ的活性成分治疗AD的潜在作用靶点,构建药物-靶标-疾病网络和蛋白与蛋白相互作用网络,运用生物信息学分析DHYZ调控凋亡网络的主要靶点和信号途径;Aβ1-42处理构建AD细胞模型,使用不同浓度含药血清处理,运用MTT法检测细胞活率,流式细胞术检测细胞凋亡情况,qPCR和Western blotting检测细胞凋亡相关基因和蛋白的表达。结果筛选出DHYZ中108种活性成分有222个AD的潜在治疗靶点,其中有202个靶点之间存在的3 737种蛋白蛋白相互作用关系,DHYZ对凋亡网络的调控...

Objective: To confirm evidence regarding the potential therapeutic effect of Dihuang Yinzi Decoction (DHYZ) on Alzheimer's disease (AD) from the regulation of apoptotic network using network pharmacology and molecular biology. Methods: The active ingredients of DHYZ were screened and the potential therapeutic targets on AD were predicted, and the drug-target-disease network and protein-protein interactions (PPI) network were built by using network pharmacology. The main targets and signaling pathways of apoptotic networks of DHYZ were performed...