期刊论文

Study on antitumor activity of triterpenoid saponins from Tujia ethnomedicine Kouziqi

Wang, W.

中文

中草药

0253-2670

2020

51

7

1831-1838

10.7501/j.issn.0253-2670.2020.07.018

湖南省自然科学基金科教联合项目(2017JJ5041) 国家自然科学基金青年基金资助项目(81703819) 湖南省大学生研究性学习和创新性实验计划项目(2018-413)。

本校为通讯机构

目的研究土家族药物扣子七Panax japonicus var. major中三萜皂苷类成分,对其抑制肿瘤细胞增殖活性进行筛选,并初步探讨化合物结构与活性的关系。方法应用多种色谱方法对土家族药物扣子七正丁醇部位进行分离,所分离化合物运用核磁共振方法进行结构鉴定,应用MTT法测定分离化合物对体外培养人肿瘤细胞增殖的影响。结果从扣子七根茎正丁醇部位分离得到14个三萜皂苷,分别鉴定为竹节参皂苷IVa甲酯(1)、竹节参皂苷IVa丁酯(2)、竹节参皂苷IV(3)、竹节参皂苷IVa(4)、28-去糖竹节参皂苷IVa(5)、齐墩果酸-3-O-β-D-(6'-甲酯)-吡喃葡萄糖醛酸苷(6)、(24R)-珠子参苷R_1(7)、(24R)-拟人参皂苷F11(8)、(20S)-三...

Objective: To study the active triterpenoid saponins of Tujia ethnomedicine Kouziqi (Panax japonicus var. major). The antitumor activity was screened and the relationship between the structure and activity of the compounds was discussed. Methods: The ethanol extract of Kouziqi was isolated by Silica gel, ODS and MCI column chromatograph and purified by preparative HPLC. The structures were elucidated on the basis of spectroscopic analysis and compared with literatures. Using MTT assay to detect the cytotoxicity of 14 compounds in BGC-823, HCT-1...