期刊论文

Maslinic acid induces autophagy through PI3K/Akt/mTOR pathway in human nasopharyngeal carcinoma cells

He, Y.-C.

中文

中草药

0253-2670

2020

51

9

2481-2485

10.7501/j.issn.0253-2670.2020.09.024

国家自然科学基金资助项目(81874408) 湖南省自然科学基金项目(2017JJ3246) 湖南省自然科学基金项目(2016JJ6117) 湖南中医药大学基础医学学科基金 湖南省教育厅优秀青年基金(19B439)。

本校为第一且通讯机构

目的研究山楂酸对鼻咽癌CNE2细胞增殖和自噬的影响,探讨PI3K/Akt/mTOR通路在该过程中的调控作用。方法采用CCK-8法检测不同浓度的山楂酸作用不同时间对CNE2细胞增殖的影响;MDC染色法检测不同浓度山楂酸处理CNE2细胞24 h后自噬小体的形成情况;Western blotting法检测山楂酸对CNE2细胞自噬相关蛋白及PI3K/AKT/mTOR通路关键蛋白表达的影响。结果与溶剂对照组相比,山楂酸可以抑制CNE2细胞的增殖,而且随药物处理时间和浓度的增加,抑制效果增强(P

Objective: To investigate the effects of maslinic acid (MA) on the proliferation and autophagy in nasopharyngeal carcinoma CNE2 cells and to elucidate the regulatory role of PI3K/Akt/mTOR pathway in this process. Methods: The effect of MA on the proliferation of CNE2 cells was assessed by CCK-8. MDC staining of autophagic vacuoles was performed for autophagy analysis. Additionally, the levels of autophagy-and PI3K/Akt/mTOR-associated proteins were examined using western blot analysis. Results: MA significantly inhibited the proliferation of CNE...