期刊论文

Effects of Zuogui Jiangtang Jieyu Formulation on insulin resistance in hipppocampus of rats with diabetes-related depression

Wang, Y.-H.

中文

中草药

0253-2670

2020

51

11

3013-3020

10.7501/j.issn.0253-2670.2020.11.020

国家自然科学基金资助项目(81603604) 湖南省自然科学基金项目(2019JJ50464) 湖南中医药大学中医学国内一流建设学科开放基金(2018ZYX46) 湖南中医药大学中医学国内一流建设学科项目(2018)。

本校为第一机构

目的 研究左归降糖解郁方对糖尿病并发抑郁症大鼠海马胰岛素抵抗的调节作用.方法 建立糖尿病并发抑郁症大鼠模型,并随机分为4组,模型组,阳性药组(二甲双胍0.18 g/kg+氟西汀1.8 mg/kg),左归降糖解郁方高、低剂量组(20.52、10.26g/kg),另设健康大鼠为对照组.各组大鼠ig给药28 d后,采用血糖仪及ELISA法检测空腹血糖及外周胰岛素抵抗程度.采用旷野实验和强迫游泳实验检测大鼠抑郁样行为.采用免疫荧光法和Western blotting法检测大鼠海马胰岛素受体磷酸化蛋白(p-IR)、磷酸化的胰岛素受体底物-1 (p-IRS-1)的表达,采用Western blotting法检测海马磷酸化的磷脂酰肌醇3-激酶(p-PI3K)、磷酸化蛋白激酶B (p...

Objective: To investigate the function of Zuogui Jiangtang Jieyu Formulation on hippocampal insulin resistance in rats with diabetes-related depression. Methods: The rat model of diabetes-related depression was established and randomly divided into four groups, including model group, positive drug group (metformin 0.18 g/kg + fluoxetine 1.8 mg/kg), high (20.53 g/kg) and low (10.26 g/kg) doses of Zuogui Jiangtang Jieyu Formulation groups. After 28 d of gavage, fasting blood glucose and peripheral insulin resistance were measured by blood glucose...