期刊论文

Anti-inflammatory effect of impressic acid from Acanthopanax gracilistylus based on NF-κB signaling pathway

Liu, X.-Q.

中文

中草药

0253-2670

2020

51

22

5781-5787

10.7501/j.issn.0253-2670.2020.22.016

湖南省自然科学基金资助项目(2019JJ40223,2019JJ50448) 湖南中医药大学生物工程重点学科资助项目(校行科字[2018]3号) 湖南省教育厅一般项目(18C0399)。

本校为第一机构

目的 研究细柱五加Acanthopanax gracilistylus W.W.Smith中impressic acid(IA)对脂多糖(LPS)诱导的小鼠单核巨噬细胞RAW264.7的抗炎作用.方法 以LPS诱导RAW264.7细胞建立炎症模型;使用EZ4U细胞增殖与细胞毒性分析试剂盒检测IA对RAW264.7细胞的毒性作用;Griess法测定一氧化氮(NO)水平;ELISA法测肿瘤坏死因子-a(TNF-a)、白介素-1β(IL-1β)水平;RT-PCR法检测TNF-α、IL-1β mRNA表达;Western blotting检测高迁移率族蛋白B1(HMGB1)蛋白表达;ELISA法细胞质和细胞核的核因子-κB(NF-κB)水平.结果 Impressic acid可以显著抑制LPS诱导的RAW264.7细胞中TNF-a、IL-1β水平和HMGB1蛋白表达,并抑制NF-κB...

Objective: To explore the anti-inflammatory effect of impressic acid (IA) from Acanthopanax gracilistylus on lipopolysaccharide (LPS)-induced RAW264.7 cells. Methods: RAW264.7 cells were stimulated by LPS to establish an inflammatory model. The cytotoxicity of IA on RAW 264.7 cells was detected by EZ4U cell proliferation and cytotoxicity analysis kit. The level of nitric oxide (NO) was determined by Griess. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were determined by ELISA. The expressions of TNF-α and IL-1β...