期刊论文

Study on general object “imprinted template” for in vitro metabolism of volatile oil from Houttuynia cordata

Zhou, Y.-Q.;He, F.-Y.

中文

中草药

0253-2670

2021

52

01

75-81

10.7501/j.issn.0253-2670.2021.01.010

国家自然科学基金资助项目（81573691）;国家自然科学基金资助项目（81874507）;国家自然科学基金青年项目（81803729）； 湖南省教育厅优秀青年项目（20B438）； 湖南省自然科学基金青年项目（2019JJ50430）；

本校为第一机构

目的基于体外代谢模型对鱼腥草Houttuynia cordata挥发油代谢产物通用客体"印迹模板"进行研究。方法自制鱼腥草注射液（Houttuyniae Injection,HI）,将其在大鼠肝微粒（RLM）中进行孵育,于不同时间点采集样本,进行GC-MS测定;计算所有代谢产物的分子连接性指数（MCI）,并从中筛选出代谢产物的共有成分和非共有成分,总结其结构特点,运用夹角余弦法计算相似度;将所有代谢成分进行归类。结果共得到鱼腥草挥发油代谢产物62个,其中共有成分6个,分别是十四烷、植烷、正癸酸、己酸、乙酸异龙脑酯和正己醇,除乙酸异龙脑酯外,MCI与总体代谢产物相似度较高（0.914～0.964）。将非共有成分归类得到6个成分群...

Objective: To study general object “imprinted template” of volatile oil metabolites of Houttuynia cordata based on in vitro metabolic model. Methods: Houttuyniae Injection was incubated in rat liver microparticle (RLM). The samples were collected at different time points for GC-MS determination. The molecular connectivity index (MCI) of all metabolites was calculated. The common and non-common components of metabolites were selected, and the structural characteristics were summarized. The similarity was calculated by angle cosine method, and ...