期刊论文

Study on community and diversity of endophyte and its correlation with active ingredient content in Polygonatum cyrtonema

Zhang, S.-H.

中文

中草药

0253-2670

2021

52

13

4023-4030

10.7501/j.issn.0253-2670.2021.13.025

国家自然科学青年基金项目（81503197）； 湖南省自然科学青年基金项目（2018JJ3310）； 湖南省中医药管理局项目（201810）； 湖南省中医药研究院自主项目（201705）； 湖南创新型省份建设科技扶贫政策性项目（2019NK3010-02）； 湖南省科技成果转化及产业化计划项目（2020SK2029）；

本校为第一机构

目的通过分析多花黄精Polygonatumcyrtonema内生菌菌群结构多样性及其与主要有效成分含量之间的相关性,探讨多花黄精内生菌对其药材品质的影响。方法收集不同产地多花黄精样品,采用高通量测序对内生菌群落结构进行分析,并测定多糖、总皂苷等有效成分含量,通过双因素关联分析探究菌群多样性及其与有效成分之间的关系。结果 5个产地15组多花黄精样品共得到1 183 635条有效序列,划分为59 474个操作分类单元（operational taxonomic units,OTU）,其中细菌分19门,47纲,120目,201科,419属;属水平上,泛菌属为优势菌群,最大占比达到61.1%。真菌分7门,27纲,70目,132科,187属;属水平上,子囊菌门一未鉴定属...

Objective: To explore the effect of endophyte on the quality of Polygonatum cyrtonema by analyzing the correlation between community and diversity of endophyte and the content of active ingredient. Methods: P. cyrtonema from different locations were collected; High-throughput sequencing technology was used to analyze endophyte and the content of active ingredient were determined, including polysaccharides, total saponins and so on. The relationship between the diversity of endophyte and the active components was explored through two-factor corr...