期刊论文

Preparation process optimization and characterization of RGD cyclopeptide modified curcumin/baicalin co-delivery targeted liposomes

Hu, L.-Z.;Gui, H.

中文

中草药

0253-2670

2021

52

22

6834-6844

10.7501/j.issn.0253-2670.2021.22.008

湖南中医药大学中药学一流学科开放基金项目（2020ZYX09）；

本校为第一且通讯机构

目的优化RGD环肽修饰的姜黄素(curcumin,Cur)/黄芩苷(baicalin,Bai)共递送靶向纳米脂质体(RGD-Cur/Bai-Lip)的制备工艺并进行表征评价。方法采用乙醇注入法制备RGD-Cur/Bai-Lip,首先通过Plackett-Burman筛选实验确定超声时间、胆脂比、水合温度作为制备RGD-Cur/Bai-Lip的关键因素,然后采用Box-Behnken响应面法进行工艺优化,以姜黄素包封率、黄芩苷包封率、总载药量为考察指标,采用AHP-复相关系数法-拉开档次法进行多指标组合赋权以确定各指标权重系数并计算综合评分,从而确定RGD-Cur/Bai-Lip的最优处方及关键工艺参数。最后对其形态、粒径电位、稳定性、体外释放度及溶血性等进行表征评价。结果...

Objective: To optimize the preparation and characterization of curcumin/baicalin co-delivery targeting nanoliposomes (RGD-Cur/Bai-Lip) modified by RGD cyclopeptide. Methods: RGD-Cur/Bai-Lip was prepared by ethanol injection. Ultrasonic time, bile-fat ratio and hydration temperature were determined by Plackett-Burman screening experiment as the key factors for the preparation of RGD-Cur/Bai-Lip. Then the process was optimized by Box-Behnken response surface method, with curcumin entrapment efficiency, baicalin entrapment efficiency and total dru...