期刊论文

Ge, J.-W.

中文

中草药

0253-2670

2022

53

23

7421-7429

10.7501/j.issn.0253-2670.2022.23.012

2020JJ5424:湖南省自然科学基金资助项目 21B30386:湖南省教育厅青年基金资助项目 2020ZXYJH08:湖南中医药大学中西医结合双一流学科开放基金资助项目

本校为第一机构

目的 基于接触系统探讨僵蚕提取液激活凝血因子XII（coagulation factor XII,FXII）对缺血性脑卒中大鼠血管新生的干预作用。方法 检测僵蚕提取液干预血浆凝血四项，体外检测僵蚕提取液对FXII及其下游底物激肽释放酶（kallikreinkinin,KK）的激活作用。通过大鼠脑缺血再灌注损伤模型，检测大鼠的脑形态学及FXII、KK、血管内皮生长因子（vascular endothelial cell growth factors,VEGF）、CD31、Brdu+/vWF+蛋白表达；通过氧糖剥夺模型，检测大鼠脑微血管内皮细胞的形态学改变、细胞增殖情况及VEGF的表达。采用LC-MS鉴定僵蚕提取液药效物质。结果 在体外实验中，僵蚕提取液延长了人血浆部分凝血酶...

Objective To explore the interventional effect of Jiangcan (Bombyx Batryticatus) extracts as an activator of coagulation factor Ⅻ (FⅫ) on angiogenesis of rats with ischemic stroke by contact system. Methods The effects of Bombyx Batryticatus extract on plasma coagulation were detected, and the activation of FⅫ and its downstream substrate kallikrein kinase (KK) by Bombyx Batryticatus extract was detected in vitro. The brain morphology and expressions of FⅫ, KK, vascular endothelial growth factors (VEGF), CD31, Brdu+/vWF+ were detected by ra...