Objective To establish HPLC characteristic fingerprint and single standard to determine multi-components methods to effectively distinguish Huangbai (Phellodendri Chinensis Cortex) from its confusing species, Phellodendri Amurense Cortex, so as to promote the efficacy and safety of Phellodendri Chinensis Cortex. Methods HPLC was used to analyze the chemical components from Phellodendri Chinensis Cortex. The chromatographic conditions were optimized, the best chromatographic conditions were determined, and then the characteristic chromatogram wa...