会议论文

中文

2015

94-95

第八届全国临床中药学学术年会

第八届全国临床中药学学术年会论文集

2015-07-22

哈尔滨

中华中医药学会

本校为第一机构

　　目的 建立同时测定细柱五加叶总苷元提取物中4种三萜苷元的高效液相色谱(HPLC)分析方法.方法 采用Phenomenex Columbus C18 110A色谱柱(250 mm×4.60 mm 5.0 m)；柱温30℃；以乙腈-0.2％磷酸水为流动相梯度洗脱检测；波长210 nm；流速1mL/min.结果 4个成分均能达到基线分离,样品含量测定出各组分在相应的浓度范围内均呈现良好线性关系,其平均加样回收率分别为97.8％,96.0％,96.4％,98.4％.结论 本方法可用于同时测定细柱五加叶总苷元提取物中 3,11α,23-trihydroxy-hp-20(29)-en-28-oic acid、3α, 11-dihydroxy-23-oxo-hp-20(29)-en-28-oic acid、impressic acid、acankoreanogein的含量,为...

　　Objective Toestablish HPLC methods for the simultaneous determination of four triterpene saponins from theleaves of Acanthopanaxgracilistylu.Methods The HPLC method was established.A Phenomenex Columbus C18 110A column(250 mim×4.60 rmm, 5.0 μm) was used for separation at temperature 30℃.The samples were gradient eluted withacetonitrile-0.1% phosphoric acid.The detection wavelength was at 210 nm and the flow rate was at 1 mL/min.Results Fourconstituents in the corresponding concentration range showed a good linear relationship.The average...