A simple, sensitive and reliable HPLC-DAD method was performed for the simultaneous determination of 13 nucleosides and nucleobases including cytosine, uracil, cytidine, hypoxanthine, uridine, thymine, 2′-deoxyuridine, adenine, inosine, guanosine, thymidine, adenosine and 2′-deoxyadenosine in Ganoderma lucidum and its related species. The analysis was conducted on a phenomenex C18 column (250 mm × 4.6 mm i.d., 5 mm) eluting with a gradient of acetonitrile in water at a flow rate of 0.6 mL/min and the detection wavelength was set as 260 nm. T...
