National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21177052]; Science and Technology Program of Hunan Province in China [2010SK3039]; Construct Program of the Key Discipline (Public Health and Preventive Medicine) in Hunan Province
机构署名：
本校为其他机构
院系归属：
中医学院
摘要：
We report on a photometric method for the determination of the metallothioneins (MTs). It is known that citrate capped gold nanoparticles (AuNPs) coated with traces of mercury possess peroxidase-like properties that can catalyze the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulfonate) (ABTS) to form a blue product in acetate buffer of pH 4.5. It is found that if the AuNPs are first aggregated by the cysteine-rich metallothioneins, the peroxidase-like properties of the resulting aggregates (AuNP-Hg-MTs) cause a largely accelerated o...
