Research Foundation of Education Bureau of Hunan Province, China [16A227]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2018JJ3869]; Science and Technology Innovation Foundation of Graduate Students in Hunan Province [CX2016B3]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21305164]
机构署名:
本校为其他机构
院系归属:
国际教育学院
摘要:
Abstract: A simple electrochemical method for detecting glutathione reductase was developed in this work. It was observed that the impedance of electrode increased obviously after the electrode modification by glutathione, and the value of impedance was closely related to the reduced glutathione (GSH) concentration. Based on the fact that glutathione reductase (GR) could catalyze oxidized glutathione (GSSG) to GSH rapidly in the presence of NADPH (β-nicotinamide adenine dinucleotide 2'-phosphate reduced), and as GSH was immobilized on the gold...