Hunan Provincial Development and Reform Commission, China
机构署名：
本校为其他机构
院系归属：
药学院
摘要：
A novel potentiometric sensing platform was designed for real-time electronic monitoring of telomerase activity using a copper(II) ion-selective electrode (Cu-ISE). The target-induced coordination of pyrophosphate ion with copper ion (Cu 2+ ) was utilized for the detection of the enzyme activity. Upon telomerase introduction, a DNA primer was elongated in the presence of deoxyribonucleoside triphosphates, accompanying formation of the by-product (pyrophosphate ion; P 2 O 7 4- ). The generated pyrophosphate ions chemically coordinated with free ...
