期刊论文

中文

现代生物医学进展

1673-6273

2014

14

26

5042-5044

10.13241/j.cnki.pmb.2014.26.011

(2011FJ3141):湖南省科技厅资助项目 (99820001-39):湖南中医药大学校青年科研课题项目 (81202632,81373702):国家自然科学基金项目

本校为第一机构

目的：从肿瘤坏死因子-a（TNF-a）和白介素（IL-1β）表达变化揭示丹龙醒脑片抗老年脑缺血再灌注损伤的保护机制。方法：用大脑中动脉栓塞再通法建立脑缺血再灌注模型，将动物随机分为假手术组、模型组、丹龙醒脑片组和尼莫地平组，采用免疫组化法，观察TNF-a 和IL-1β蛋白的表达。结果：丹龙醒脑片能显著减轻神经细胞的损伤；假手术组有一定量的TNF-a 和IL-1β表达，缺血后TNF-a 和IL-1β迅速增加，丹龙醒脑片组显著抑制TNF-a 和IL-1β的表达，模型组和丹龙醒脑片组差异有统计学意义（P〈0.01,P〈0.05）。结论：丹龙醒脑片抗老年脑缺血再灌注损伤的保护机制与其抑制由缺血再灌注损伤诱导的TNF-...

Objective： To investigate protective mechanism of Danlong Xingnao tablets through the change of expression of TNF-a and IL-I[3 after cerebral ischemia-reperfusion injury in elderly rats. Methods： An animal model of cerebral ischemia-reperfusion was made by middle cerebral artery occlusion-recanalization. Rats were randomly divided into sham-operation group,model group, Danlong Xingnao tablet group and nimodipine group. The expression of TNF-a and IL-1β were detected by immunohistochemical method and image analysis technique. Results： Danlon...