期刊论文

Wang Yu-Hong

中文

数字中医药(英文)

2096-479X

2020

3

2

80-95

10.1016/j.dcmed.2020.06.003

We thank for the funding support from the National Major New Drug Development Project (No. 2017ZX09309026) and Provincial Department of Graduate Research Innovation Project of Hunan (No. CX20190565).

本校为第一且通讯机构

目的 探讨复方柴金解郁片(CJJYT)对抑郁症认知障碍的改善作用及其潜在机制.方法 体外实验中,从胎鼠全脑中分离海马,进一步培养海马神经元细胞;实验前18 h,除对照组外,每组均加50μM CORT构建抑郁环境;建立模型后,空白血清组加10％空白血清,CJJYT组、文拉法辛组分别加入对应10％含药血清,空白组和模型组则加入等体积培养液.检测各组海马神经元细胞内Ca2+平均荧光强度;记录mEPSC电流幅度和频率,评估海马神经元的突触功能.此外,通过免疫荧光染色,高内涵分析海马神经元突触可塑性相关蛋白SYN-α、NR2A、NR2B、PSD-95、CaMKⅡ和SynGAP的表达;采用反转录聚合酶链反应检测SYN-α、NR2A、NR2B、PSD-95、...

Objective: To investigate the effectiveness of compound Chai Jin Jie Yu Tablets (CJJYT) in ameliorating cognitive impairment associated with depression and its potential mechanism of action. Methods: In vitro experiments, the hippocampus was isolated from the whole brain of the fetal rat and cultured into hippocampal neuron cells. 50 μM corticosterone (CORT) was added to each group 18 h before the experiment for modeling depression, with the exception of the control group. After modeling, the blank serum group was added with 10% blank serum, t...