期刊论文

中文

中医药导报

1672-951X

2010

16

6

106-108

10.13862/j.cnki.cn43-1446/r.2010.06.055

本校为第一机构

目的:检测生脉注射液不同制备工艺中间体残留蛋白质含量.方法:以残留蛋白质为检测目标,采用透析法获取生脉注射液不同制备工艺中间体中大分子杂质,Bradford法测定各大分子杂质中残留蛋白质含量,结合SDS-PAGE电泳分析各残留蛋白质分子量.结果:在0-8 μg线性范围内,牛血清白蛋白标准品回归方程为:Y=0.0474X-0.003(r=0.9974);不同工艺对提取液中残留蛋白的影响较大,红参提取液含残留蛋白浓度较高;麦冬、五味子提取液残留蛋白含量相对较低;经对SDS-PAGE电泳结果分析,各不同工艺提取液中间体含蛋白质分子量主要集中于6-11万.结论:生脉注射液中间体提取工艺工程中可能有蛋白质残留,应加强残留蛋白质检...

Objective：Shengmai injection intermediate of different processes residual protein content.Methods：The target for the detection of residual protein,using dialysis for Health and Injection Preparation of intermediates in the macromolecules of different impurities,Bradford Determination of major elements in the residual protein content of impurities,combined with SDS-PAGE electrophoresis analysis of the residues in the protein molecular weight.Results：The linear range of 0-8 μg,bovine serum albumin standard regression equation：Y=0.0474X-0.003...