目的：建立同时测定疏毛吴茱萸中吴茱萸碱、吴茱萸次碱和柠檬苦素的含量的方法以及分析含量动态变化,为合理采收提供科学依据。方法：采用HPLC法,色谱柱：Agi Lent C18（4.6 mm×250 mm,5μm）;流动相：乙腈-水（55：45）;流速：1 m L·min-1;检测波长：225 nm;柱温：25℃。结果：疏毛吴茱萸中吴茱萸碱和吴茱萸次碱的含量在7月底即果实呈黄绿色未开裂前最高,柠檬苦素含量从6月初开始逐渐递减。结论：疏毛吴茱萸的最佳采收期为果实呈黄绿色未开裂前。
摘要(英文)：
Objective： To find out the best ilarvest time for fi＇uits of Evodia rutaecarpa （var.） bodinieri by determining the contents of Evodiamine, rutaecarpine and Limonin in the fruits collected at various periods. Methods： HPLC method was applied; The separation was performed on the AgiLent C18 （4.6 mm×250 mm, 5μm）coLumn with acetonitrile-water（55：45） as mobile phase. The fLow rate was 1 mL·min^-1. the wavelength of UV detector was set at 225 nm and the column temperature was set at 25 ℃. Results： The content of evodiamine and rutaeear...
