期刊论文

中文

河南中医

1003-5028

2015

21

14

19-20

10.13862/j.cnki.cn43-1446/r.2015.14.006

09B074:湖南省教育厅科学研究项目 ZYFT201304:湖南省中药粉体与创新药物研究省部共建国家重点实验室培育基地开放基金

本校为第一机构

目的：研究护心康对My D88和TRAF6的影响,探讨其抗动脉粥样硬化的机制。方法：采用雄性中国本兔14只,随机分为正常组和护心康组,分别以蒸馏水和护心康连续灌胃7 d,末次灌胃给药2 h后心脏采血分离血清。体外培养人脐静脉内皮细胞,随机分为3组：正常组,模型组,护心康组。用LPS刺激2 h后,模型组给予空白血清,护心康组给予含药血清干预24 h,收集细胞,用荧光定量PCR和Western blot分别测定My D88和TRAF6m RNA的表达。结果：LPS刺激引起My D88和TRAF6基因表达升高（P〈0.01）;护心康组表达较模型组低（P〈0.05）。结论：护心康具有降低内皮细胞My D88和TRAF6基因表达的作用,其抗动脉粥样硬化的作用可能...

Objective： To explore the mechanism of Huxinkang on atherosclerosis rabbit induced by MyD88 and TRAF-6. Method： The 14 male Chinese white rabbits were randomly divided into 2 groups： the normal group and the Huxinkang group. Distilled water was given to the normal group, and Huxinkang was given to the Huxinkang group for 7 days. The blood was withdrawn from the heart 2 h after the last gastrogavage. The serum was isolated after centdfuge. Human umbilical vein endothe- lial cell （HUVECS） was cultured and then randomly divided into 3 groups：...